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Journal of Biomedical Optics • Open Access

Intrinsic tumor biomarkers revealed by novel double-differential spectroscopic analysis of near-infrared spectra
Author(s): Shwayta Kukreti; Albert E. Cerussi; Bruce Jason Tromberg; Enrico Gratton

Paper Abstract

We develop a double-differential spectroscopic analysis method for broadband near-infrared (NIR, 650 to 1000 nm) absorption spectra. Application of this method to spectra of tumor-containing breast tissue reveals specific cancer biomarkers. In this method, patient-specific variations in molecular composition are removed by using the normal tissue as an internal control. The effects of concentration differences of the four major tissue absorbers (oxyhemoglobin, deoxyhemoglobin, water, and bulk lipid) between the tumor and normal tissue are accounted for to reveal small spectral components unique to cancer. From a pilot study of 15 cancer patients, we find these spectral components to be characterized by specific NIR absorption bands. Based on the spectral regions of absorption at about 760, 930, and 980 nm, we identify these biomarkers with changes in state or addition of lipid and/or water. To quantify spectral variation in the absorption bands, we construct the specific tumor component (STC) index. The STC index identifies regions of the breast with tumors.

Paper Details

Date Published: 1 March 2007
PDF: 3 pages
J. Biomed. Opt. 12(2) 020509 doi: 10.1117/1.2709701
Published in: Journal of Biomedical Optics Volume 12, Issue 2
Show Author Affiliations
Shwayta Kukreti, Beckman Laser Institute and Medical Clinic (United States)
Albert E. Cerussi, Beckman Laser Institute and Medical Clinic (United States)
Bruce Jason Tromberg, Beckman Laser Institute and Medical Clinic (United States)
Enrico Gratton, Beckman Laser Institute and Medical Clinic (United States)

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