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Journal of Biomedical Optics

Hybrid phosphorescence and fluorescence native spectroscopy for breast cancer detection
Author(s): Alexandra N. Alimova; Alvin Katz; Vidyasagar Sriramoju; Yuri Budansky; Alexei A. Bykov; Roman Zelikovitch; Robert R. Alfano
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Paper Abstract

Fluorescence and phosphorescence measurements are performed on normal and malignant ex vivo human breast tissues using UV LED and xenon lamp excitation. Tryptophan (trp) phosphorescence intensity is higher in both normal glandular and adipose tissue when compared to malignant tissue. An algorithm based on the ratio of trp fluorescence intensity at 345 nm to phosphorescence intensity at 500 nm is successfully used to separate normal from malignant tissue types. Normal specimens consistently exhibited a low I345/I500 ratio (<10), while for malignant specimens, the I345/I500 ratio is consistently high (>15). The ratio analysis correlates well with histopathology. Intensity ratio maps with a spatial resolution of 0.5 mm are generated in which local regions of malignancy could be identified.

Paper Details

Date Published: 1 January 2007
PDF: 6 pages
J. Biomed. Opt. 12(1) 014004 doi: 10.1117/1.2437139
Published in: Journal of Biomedical Optics Volume 12, Issue 1
Show Author Affiliations
Alexandra N. Alimova, City College/CUNY (United States)
Alvin Katz, City College/CUNY (United States)
Vidyasagar Sriramoju, City College/CUNY (United States)
Yuri Budansky, City College/CUNY (United States)
Alexei A. Bykov, City College/CUNY (United States)
Roman Zelikovitch, City College/CUNY (United States)
Robert R. Alfano, City College/CUNY (United States)

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