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Journal of Biomedical Optics

Fluorescence polarization of tetracycline derivatives as a technique for mapping nonmelanoma skin cancers
Author(s): Anna N. Yaroslavsky; Elena Vladimirovna Salomatina; Victor Neel; Richard Rox Anderson; Thomas J. Flotte
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Paper Abstract

Nonmelanoma skin cancer is the most common form of human cancer, often resulting in high morbidity. Low visual contrast of these tumors makes their delineation a challenging problem. Employing a linearly polarized monochromatic light source and a wide-field CCD camera, we have developed a technique for fluorescence polarization imaging of the nonmelanoma cancers stained using antibiotics from the tetracycline family. To determine the feasibility of the method, fluorescence polarization images of 86 thick, fresh cancer excisions were studied. We found that the level of endogenous fluorescence polarization was much lower than that of exogenous, and that the average values of fluorescence polarization of tetracycline derivatives were significantly higher in cancerous as compared to normal tissue. Out of 86 tumors [54 stained in demeclocycline (DMN) and 32 in tetracycline (TCN)], in 79 cases (51—DMN, 28—TCN) the location, size, and shape of the lesions were identified accurately. The results of this trial indicate that nonmelanoma skin tumors can be distinguished from healthy tissue based on the differences in exogenous fluorescence polarization of TCN and/or DMN. Therefore, the developed technique can provide an important new tool for image-guided cancer surgery.

Paper Details

Date Published: 1 January 2007
PDF: 9 pages
J. Biomed. Opt. 12(1) 014005 doi: 10.1117/1.2435710
Published in: Journal of Biomedical Optics Volume 12, Issue 1
Show Author Affiliations
Anna N. Yaroslavsky, Massachusetts General Hospital (United States)
Elena Vladimirovna Salomatina, Massachusetts General Hospital (United States)
Victor Neel, Massachusetts General Hospital (United States)
Richard Rox Anderson, Wellman Ctr. for Photomedicine (United States)
Thomas J. Flotte, Massachusetts General Hospital (United States)

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