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Journal of Biomedical Optics

High-resolution whole organ imaging using two-photon tissue cytometry
Author(s): Timothy Ragan; Jeremy D. Sylvan; Ki H. Kim; Hayden Huang; Karsten Bahlmann; Richard T. Lee; Peter T. C. So
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Paper Abstract

Three-dimensional (3-D) tissue imaging offers substantial benefits to a wide range of biomedical investigations from cardiovascular biology, diabetes, Alzheimer's disease to cancer. Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated histological sectioning, which can quantify tissue morphology and physiology throughout entire organs with subcellular resolution. Furthermore, two-photon tissue cytometry offers all the benefits of fluorescence-based approaches including high specificity and sensitivity and appropriateness for molecular imaging of gene and protein expression. We use two-photon tissue cytometry to image an entire mouse heart at subcellular resolution to quantify the 3-D morphology of cardiac microvasculature and myocyte morphology spanning almost five orders of magnitude in length scales.

Paper Details

Date Published: 1 January 2007
PDF: 9 pages
J. Biomed. Opt. 12(1) 014015 doi: 10.1117/1.2435626
Published in: Journal of Biomedical Optics Volume 12, Issue 1
Show Author Affiliations
Timothy Ragan, Massachusetts Institute of Technology (United States)
Jeremy D. Sylvan, Brigham and Women's Hospital (United States)
Ki H. Kim, Massachusetts General Hospital (United States)
Hayden Huang, Brigham and Women's Hospital (United States)
Karsten Bahlmann, Massachusetts Institute of Technology (United States)
Richard T. Lee, Brigham and Women's Hospital (United States)
Peter T. C. So, Massachusetts Institute of Technology (United States)

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