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Journal of Biomedical Optics

Evaluation of dermal thermal damage by multiphoton autofluorescence and second-harmonic-generation microscopy
Author(s): Ming-Gu Lin; Tsung-Lin Yang; Cheng-Tien Chiang; Hsien-Ching Kao; Jin-Ning Lee; Wen Lo; Shiou-Hwa Jee; Yang-Fang Chen; Chen-Yuan Dong; Sung-Jan Lin
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Paper Abstract

We attempt to characterize the degree of skin thermal damage by using multiphoton microscopy to characterize dermal thermal damage. Our results show that dermal collagen and elastic fibers display different susceptibility to thermal injury. Morphologically, dermal collagen starts to denature at 60°C while fracture and aggregation of elastic fibers do not occur until 65°C. With increasing temperatures, the structures of both elastic and collagen fibers deteriorate. While second-harmonic-generation (SHG) imaging is helpful in identifying the denaturation temperature of collagen, autofluorescence (AF) imaging can help to identify the structural alternations of tissue at higher temperatures when SHG signals have decayed. We also employ a ratiometric approach based on the AF-to-SHG index of dermis (ASID) to characterize the degree of dermal thermal damage. Use of the ASID index can bypass the difficulty in analyzing inhomogeneous dermal fibers and show that dermal collagen starts to denature at 60°C. Our results suggest that with additional developments, multiphoton microscopy has potential to be developed into an effective in vivo imaging technique to monitor and characterize dermal thermal damage.

Paper Details

Date Published: 1 November 2006
PDF: 6 pages
J. Biomed. Opt. 11(6) 064006 doi: 10.1117/1.2405347
Published in: Journal of Biomedical Optics Volume 11, Issue 6
Show Author Affiliations
Ming-Gu Lin, National Taiwan Univ. (Taiwan)
Tsung-Lin Yang, National Taiwan Univ. (Taiwan)
Cheng-Tien Chiang, National Taiwan Univ. (Taiwan)
Hsien-Ching Kao, National Taiwan Univ. (Taiwan)
Jin-Ning Lee, National Taiwan Univ. (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Shiou-Hwa Jee, National Taiwan Univ. Hospital (Taiwan)
Yang-Fang Chen, National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Sung-Jan Lin, National Taiwan Univ. Hospital (Taiwan)

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