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Journal of Biomedical Optics

Method used to measure interaction of proteins with dual-beam optical tweezers
Author(s): E. Qu; Honglian Guo; Chunhua Xu; Chunxiang Liu; Zhaolin Li; Bing-Ying Cheng; Daozhong Zhang
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Paper Abstract

In the force measurement of protein-protein interaction, proteins are usually attached to microbeads, so the coated beads serve as both handles and force transducers. Due to the short interaction distance between proteins, the beads are usually close enough to each other. When dual-beam optical tweezers and quadrant photodiode detector are used to investigate the interaction of proteins, it is found that the signal of detected beads is greatly affected by adjacent beads. Analysis reveals that the contribution of two beads to the quadrant detector signal is independent. A method for extracting the real interaction signal from a disturbed one is presented. Based on this method, interaction between microtubules and AtMAP65-1 is measured. The results show that this method is useful for measuring short-distance interaction with the precision of piconewton and nanometer scales.

Paper Details

Date Published: 1 November 2006
PDF: 6 pages
J. Biomed. Opt. 11(6) 064035 doi: 10.1117/1.2397575
Published in: Journal of Biomedical Optics Volume 11, Issue 6
Show Author Affiliations
E. Qu, Institute of Physics (China)
Honglian Guo, Institute of Physics (China)
Chunhua Xu, Institute of Physics (China)
Chunxiang Liu, Institute of Physics (China)
Zhaolin Li, Institute of Physics (China)
Bing-Ying Cheng, Institute of Physics (China)
Daozhong Zhang, Institute of Physics (China)

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