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Journal of Biomedical Optics

Noninvasive harmonics optical microscopy for long-term observation of embryonic nervous system development in vivo
Author(s): Szu-Yu Chen; Cho-Shuen Hsieh; Shi-Wei Chu; Cheng-Yung Lin; Ching-Yi Ko; Yi-Chung Chen; Huai-Jen Tsai; Chin-Hwa Hu; Chi-Kuang Sun
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Paper Abstract

Nervous system development is a complicated dynamic process, and many mechanisms remain unknown. By utilizing endogenous second-harmonic-generation as the contrast of polarized nerve fibers and third-harmonic-generation (THG) to reveal morphological changes, we have successfully observed the vertebrate embryonic nervous development from the very beginning based on a 1230-nm light source. The dynamic development of the nerve system within a live zebrafish embryo can be recorded continuously more than 20 hr without fluorescence markers. Since the THG process is not limited by the time of gene expression and differentiation as fluorescence-based techniques are, the observable stages can be advanced to the very beginning of the development process. The complete three-dimensional brain development from a neural plate to a neural tube can be uncovered with a submicron lateral resolution. We have, for the first time, also reported the generation of SHG from myelinated nerve fibers and the outer segment of the photoreceptors with a stacked membrane structure. Our study clearly indicates the fact that higher-harmonics-based optical microscopy has the strong potential to long-term in vivo study of the nervous system, including genetic disorders of the nervous system, axon pathfinding, neural regeneration, neural repair, and neural stem cell development.

Paper Details

Date Published: 1 September 2006
PDF: 8 pages
J. Biomed. Opt. 11(5) 054022 doi: 10.1117/1.2363369
Published in: Journal of Biomedical Optics Volume 11, Issue 5
Show Author Affiliations
Szu-Yu Chen, National Taiwan Univ. (Taiwan)
Cho-Shuen Hsieh, National Taiwan Univ. (Taiwan)
Shi-Wei Chu, National Taiwan Univ. (Taiwan)
Cheng-Yung Lin, National Taiwan Univ. (Taiwan)
Ching-Yi Ko, National Taiwan Ocean Univ. (Taiwan)
Yi-Chung Chen, National Taiwan Ocean Univ. (Taiwan)
Huai-Jen Tsai, National Taiwan Univ. (Taiwan)
Chin-Hwa Hu, National Taiwan Ocean Univ. (Taiwan)
Chi-Kuang Sun, National Taiwan Univ. (Taiwan)

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