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Journal of Biomedical Optics

Use of glycerol as an optical clearing agent for enhancing photonic transference and detection of Salmonella typhimurium through porcine skin
Author(s): Keesla Moulton; Frank Lovell; Ellen Williams; Peter Ryan; Donald C. Lay; Eric Duco Jansen; Scott T. Willard
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Paper Abstract

The objective of this study was to evaluate glycerol (GLY) and GLY + dimethyl sulfoxide (DMSO) to increase photonic detection of transformed Salmonella typhimurium (S. typh-lux) through porcine skin. Skin was placed on 96-well plates containing S. typh-lux, imaged (5 min) using a CCD camera, and then completely immersed in PBS, GLY, DMSO, GLY+DMSO in a dose- and time-dependent manner and re-imaged (5 min). The percent of photonic emissions detected (treated or untreated skin relative to no skin controls) was used for analysis. Treatment for 4 h with 50% GLY-PBS and 50:30:20% GLY:DMSO:PBS increased photonic detection compared to untreated skin, 100% PBS, or 30:70% DMSO:PBS. Treatment with 50% GLY in the presence of 20 and 40% DMSO (v/v with PBS) increased photonic detection compared to 50% GLY alone and in the presence of 10% DMSO: 50% GLY (v/v with PBS). Data indicate that GLY and GLY+DMSO are effective optical clearing agents on porcine skin (2–3 mm thick) when treated for 4 h to increase detection of emitted photons. Clearing agents such as GLY have the potential to minimize effects of porcine skin tissue as one of the photon transmittance barriers (i.e., skin, fat, muscle, and visceral tissues) in vivo.

Paper Details

Date Published: 1 September 2006
PDF: 8 pages
J. Biomed. Opt. 11(5) 054027 doi: 10.1117/1.2363366
Published in: Journal of Biomedical Optics Volume 11, Issue 5
Show Author Affiliations
Keesla Moulton, The Univ. of Mississippi (United States)
Frank Lovell, The Univ. of Mississippi (United States)
Ellen Williams, The Univ. of Mississippi (United States)
Peter Ryan, The Univ. of Mississippi (United States)
Donald C. Lay, U.S. Dept. of Agriculture (United States)
Eric Duco Jansen, Vanderbilt Univ. (United States)
Scott T. Willard, Mississippi State Univ. (United States)

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