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Journal of Biomedical Optics • Open Access

Biochemical analysis of human breast tissues using Fourier-transform Raman spectroscopy
Author(s): Renata Andrade Bitar; Herculano da Silva Martinho; Carlos Julio Tierra-Criollo; Leandra Náira Zambelli Ramalho; Mario Mourão Netto; Airton Abrahão Martin

Paper Abstract

We employ Fourier–transform Raman spectroscopy to study normal and tumoral human breast tissues, including several subtypes of cancers. We analyzed 194 Raman spectra from breast tissues that were separated into 9 groups according to their corresponding histopathological diagnosis. The assignment of the relevant Raman bands enabled us to connect the several kinds of breast tissues (normal and pathological) to their corresponding biochemical moieties alterations and distinguish among 7 groups: normal breast, fibrocystic condition, duct carcinoma in situ, duct carcinoma in situ with necrosis, infiltrating duct carcinoma not otherwise specified, colloid infiltrating duct carcinoma, and invasive lobular carcinomas. We were able to establish the biochemical basis for each spectrum, relating the observed peaks to specific biomolecules that play a special role in the carcinogenesis process. This work is very useful for the premature optical diagnosis of a broad range of breast pathologies. We noticed that we were not able to differentiate inflammatory and medullary duct carcinomas from infiltrating duct carcinoma not otherwise specified.

Paper Details

Date Published: 1 September 2006
PDF: 8 pages
J. Biomed. Opt. 11(5) 054001 doi: 10.1117/1.2363362
Published in: Journal of Biomedical Optics Volume 11, Issue 5
Show Author Affiliations
Renata Andrade Bitar, Univ. do Vale do Paraíba (Brazil)
Herculano da Silva Martinho, Univ. do Vale do Paraíba (Brazil)
Carlos Julio Tierra-Criollo, Federal Univ. of Minaz Gerais (Brazil)
Univ. do Vale do Paraíba (Brazil)
Leandra Náira Zambelli Ramalho, Univ. of Sao Paulo (Brazil)
Mario Mourão Netto, Hospital do Câncer-A.C. Camargo (Brazil)
Airton Abrahão Martin, Univ. do Vale do Paraíba (Brazil)

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