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Journal of Biomedical Optics

Improving the penetration depth in multiphoton excitation laser scanning microscopy
Author(s): Gail McConnell
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Paper Abstract

More than a threefold increase in multiphoton laser scanning microscopy depth penetration using a passive predispersion compensation system is reported. Using dispersion-controlled pulses to counteract the effects of positive group delay dispersion in the imaging platform, optical sectioning of fluorescent samples to depths in excess of 800 µm was observed, compared with only 240 µm using a noncompensated setup. Experimental results obtained from both the predispersion compensated and noncompensated systems are compared with theoretical values of pulse broadening in a laser scanning microscope. The observed improvement in depth profiling potentially widens the applications and user base of nonlinear microscopy techniques.

Paper Details

Date Published: 1 September 2006
PDF: 7 pages
J. Biomed. Opt. 11(5) 054020 doi: 10.1117/1.2360593
Published in: Journal of Biomedical Optics Volume 11, Issue 5
Show Author Affiliations
Gail McConnell, Univ. of Strathclyde (United Kingdom)

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