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Journal of Biomedical Optics

Quantum dot optical encoded polystyrene beads for DNA detection
Author(s): Yuan-Cheng Cao; Tian-Cai Liu; Xiao-Feng Hua; Xiao-Xia Zhu; Hai-Qiao Wang; Zhen-Li Huang; Yuan-Di Zhao; Man-Xi Liu; Qingming Luo
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Paper Abstract

A novel multiplex analysis technology based on quantum dot (QD) optical encoded beads was studied. Carboxyl functionalized polystyrene beads, about 100 µm in size, were precisely encoded by the various ratios of two types of QDs whose emission wavelengths are 576 and 628 nm, respectively. Then the different encoded beads were covalently immobilized with different probes in the existing of sulfo-NHS and 1-[3-(Dimethylamino) propyl]-3-ethylcarbodiimide methiodide, and the probe density could reach to 3.1 mmol/g. These probe-linked encoded beads were used to detect the target DNA sequences in complex DNA solution by hybridization. Hybridization was visualized using fluorescein isothiocynate–labeled DNA sequences. The results show that the QDs and target signals can be obviously identified from a single-bead-level spectrum. This technology can detect DNA targets effectively with a detection limit of 0.2 µg/mL in complex solution.

Paper Details

Date Published: 1 September 2006
PDF: 7 pages
J. Biomed. Opt. 11(5) 054025 doi: 10.1117/1.2358957
Published in: Journal of Biomedical Optics Volume 11, Issue 5
Show Author Affiliations
Yuan-Cheng Cao, Huazhong Univ. of Science and Technology (China)
Tian-Cai Liu, Huazhong Univ. of Science and Technology (China)
Xiao-Feng Hua, Huazhong Univ. of Science and Technology (China)
Xiao-Xia Zhu, Huazhong Univ. of Science and Technology (China)
Hai-Qiao Wang
Zhen-Li Huang, Huazhong Univ. of Science and Technology (China)
Univ. of Central Florida (United States)
Yuan-Di Zhao, Huazhong Univ. of Science and Technology (China)
Man-Xi Liu, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)


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