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Journal of Biomedical Optics

Computer-based tracking of single sperm
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Paper Abstract

This paper describes a robust single sperm tracking algorithm (SSTA) that can be used in laser optical trapping and sperm motility studies. The algorithm creates a region of interest (ROI) centered about a sperm selected by the user. SSTA contrast enhances the ROI image and implements a modified four-class thresholding method to extract the tracked sperm as it transitions in and out of focus. The nearest neighbor method is complemented with a speed-check feature to aid tracking in the presence of additional sperm or other particles. SSTA has a collision-detection feature for real or perceived collision or near-miss cases between two sperm. Subsequent postcollision analysis employs three criteria to distinguish the tracked sperm in the image. The efficacy of SSTA is validated through examples and comparisons to commercially available computer-aided sperm tracking systems.

Paper Details

Date Published: 1 September 2006
PDF: 10 pages
J. Biomed. Opt. 11(5) 054009 doi: 10.1117/1.2357735
Published in: Journal of Biomedical Optics Volume 11, Issue 5
Show Author Affiliations
Linda Z. Shi, Univ. of California/San Diego (United States)
Jaclyn Nascimento, Univ. of California/San Diego (United States)
Michael W. Berns, Univ. of California/Irvine (United States)
Elliot L. Botvinick, Univ. of California/Irvine (United States)


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