Share Email Print
cover

Journal of Biomedical Optics

Dehydration mechanism of optical clearing in tissue
Author(s): Christopher G. Rylander; Oliver F. Stumpp; Thomas E. Milner; Nathaniel Joseph Kemp; John M. Mendenhall; Kenneth R. Diller; Ashley J. Welch
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Previous studies identified various mechanisms of light scattering reduction in tissue induced by chemical agents. Our results suggest that dehydration is an important mechanism of optical clearing in collagenous and cellular tissue. Photographic and optical coherence tomography images indicate that air-immersed skin and tendon specimens become similarly transparent to glycerol-immersed specimens. Transmission electron microscopy images reveal that dehydration causes individual scattering particles such as collagen fibrils and organelles to become more densely packed, but does not significantly alter size. A heuristic particle-interaction model predicts that the scattering particle volume fraction increase can contribute substantially to optical clearing in collagenous and cellular tissue.

Paper Details

Date Published: 1 July 2006
PDF: 7 pages
J. Biomed. Opt. 11(4) 041117 doi: 10.1117/1.2343208
Published in: Journal of Biomedical Optics Volume 11, Issue 4
Show Author Affiliations
Christopher G. Rylander, The Univ. of Texas/Austin (United States)
Oliver F. Stumpp, Reliant Technologies Inc. (United States)
Thomas E. Milner, The Univ. of Texas/Austin (United States)
Nathaniel Joseph Kemp, The Univ. of Texas/Austin (United States)
John M. Mendenhall, The Univ. of Texas/Austin (United States)
Kenneth R. Diller, The Univ. of Texas/Austin (United States)
Ashley J. Welch, The Univ. of Texas/Austin (United States)


© SPIE. Terms of Use
Back to Top