Share Email Print

Journal of Biomedical Optics

Linear response range characterization and in vivo application of laser speckle imaging of blood flow dynamics
Author(s): Bernard Choi; Julio C. Ramírez-San-Juan; Justin Lotfi; J. Stuart Nelson
Format Member Price Non-Member Price
PDF $20.00 $25.00

Paper Abstract

Noninvasive blood flow imaging can provide critical information on the state of biological tissue and the efficacy of approaches to treat disease. With laser speckle imaging (LSI), relative changes in blood flow are typically reported, with the assumption that the measured values are on a linear scale. A linear relationship between the measured and actual flow rate values has been suggested. The actual flow rate range, over which this linear relationship is valid, is unknown. Herein we report the linear response range and velocity dynamic range (VDR) of our LSI instrument at two relevant camera integration times. For integration times of 1 and 10 ms, the best case VDR was 80 and 60 dB, respectively, and the worst case VDR was 20 and 50 dB. The best case VDR values were similar to those reported in the literature for optical Doppler tomography. We also demonstrate the potential of LSI for monitoring blood flow dynamics in the rodent dorsal skinfold chamber model. These findings imply that LSI can provide accurate wide-field maps of microvascular blood flow rate dynamics and highlight heterogeneities in flow response to the application of exogenous agents.

Paper Details

Date Published: 1 July 2006
PDF: 7 pages
J. Biomed. Opt. 11(4) 041129 doi: 10.1117/1.2341196
Published in: Journal of Biomedical Optics Volume 11, Issue 4
Show Author Affiliations
Bernard Choi, Beckman Laser Institute and Medical Clinic (United States)
Julio C. Ramírez-San-Juan, Instituto Nacional de Astrofísica, Óptica y Electrónica (Mexico)
Justin Lotfi, Univ. of California/Irvine (United States)
J. Stuart Nelson, Univ. of California/Irvine (United States)

© SPIE. Terms of Use
Back to Top