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Journal of Biomedical Optics

Fluorescence-enhanced optical tomography of a large tissue phantom using point illumination geometries
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Paper Abstract

We demonstrate fluorescence-enhanced optical imaging of single and multiple fluorescent targets within a large (~1081 cm3) phantom using frequency-domain photon migration measurements of fluorescence collected at individual points in response to illumination of excitation light at individual points on the boundary. The tissue phantom was filled with a 1% lipid solution with and without 0.01 µM Indocyanine Green (ICG) and targets consisted of vials filled with the 1% lipid containing 1–2.5 µM ICG. Measurements were acquired using a modulated intensified CCD imaging system under different experimental conditions. For 3-D image reconstruction, the gradient-based penalty modified barrier function (PMBF) method with simple bounds constrained truncated Newton with trust region method (CONTN) was used. Targets of 0.5, 0.6, and 1.0 cm3 at depths of 1.4–2.8 cm from the phantom surface were tomographically reconstructed. This work demonstrates the practicality of fluorescence-enhanced tomography in clinically relevant volumes.

Paper Details

Date Published: 1 July 2006
PDF: 17 pages
J. Biomed. Opt. 11(4) 044007 doi: 10.1117/1.2338807
Published in: Journal of Biomedical Optics Volume 11, Issue 4
Show Author Affiliations
Ranadhir Roy, Baylor College of Medicine (United States)
Anuradha Godavarty, Florida International Univ. (United States)
Eva Marie Sevick-Muraca, Baylor College of Medicine (United States)

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