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Journal of Biomedical Optics

Doppler optical coherence tomography to monitor the effect of photodynamic therapy on tissue morphology and perfusion
Author(s): Maurice C. G. Aalders; Martijn L. Triesscheijn; Marjan Ruevekamp; Daniel Martijn de Bruin; Paul Baas; Dirk J. Faber; Fiona A. Stewart
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Paper Abstract

We investigated the feasibility of using optical coherence tomography (OCT) for noninvasive real-time visualization of the vascular effects of photodynamic therapy (PDT) in normal and tumor tissue in mice. Perfusion control measurements were initially performed after administrating vaso-active drugs or clamping of the subcutaneous tumors. Subsequent measurements were made on tumor-bearing mice before and after PDT using the photosensitizer meta-tetrahydroxyphenylchlorin (mTHPC). Tumors were illuminated using either a short drug light interval (D-L, 3h), when mTHPC is primarily located in the tumor vasculature or a long D-L interval (48 h), when the drug is distributed throughout the whole tumor. OCT enabled visualization of the different layers of tumor, and overlying skin with a maximal penetration of ≤ 0.5–1 mm. PDT with a short D-L interval resulted in a significant decrease of perfusion in the tumor periphery, to 20% of pre-treatment values at 160 min, whereas perfusion in the skin initially increased by 10% (at 25 min) and subsequently decreased to 60% of pre-treatment values (at 200 min). PDT with a long D-L interval did not induce significant changes in perfusion. The concept of using noninvasive OCT measurements for monitoring early, treatment-related changes in morphology and perfusion may have applications in evaluating effects of anti-angiogenic or antivascular (cancer) therapy.

Paper Details

Date Published: 1 July 2006
PDF: 8 pages
J. Biomed. Opt. 11(4) 044011 doi: 10.1117/1.2337302
Published in: Journal of Biomedical Optics Volume 11, Issue 4
Show Author Affiliations
Maurice C. G. Aalders, Academisch Medisch Ctr. (Netherlands)
Martijn L. Triesscheijn, Netherlands Cancer Institute (Netherlands)
Marjan Ruevekamp, Netherlands Cancer Institute (Netherlands)
Daniel Martijn de Bruin, Massachusetts General Hospital (United States)
Paul Baas, Netherlands Cancer Institute (Netherlands)
Dirk J. Faber, Academisch Medisch Ctr. (Netherlands)
Fiona A. Stewart, Netherlands Cancer Institute (Netherlands)


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