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Journal of Biomedical Optics • Open Access

Three-dimensional imaging of skin melanoma in vivo by dual-wavelength photoacoustic microscopy

Paper Abstract

Dual-wavelength reflection-mode photoacoustic microscopy is used to noninvasively obtain three-dimensional (3-D) images of subcutaneous melanomas and their surrounding vasculature in nude mice in vivo. The absorption coefficients of blood and melanin-pigmented melanomas vary greatly relative to each other at these two optical wavelengths (764 and 584 nm). Using high-resolution and high-contrast photoacoustic imaging in vivo with a near-infrared (764-nm) light source, the 3-D melanin distribution inside the skin is imaged, and the maximum thickness of the melanoma (~0.5 mm) is measured. The vascular system surrounding the melanoma is also imaged with visible light (584 nm) and the tumor-feeding vessels found. This technique can potentially be used for melanoma diagnosis, prognosis, and treatment planning.

Paper Details

Date Published: 1 May 2006
PDF: 4 pages
J. Biomed. Opt. 11(3) 034032 doi: 10.1117/1.2210907
Published in: Journal of Biomedical Optics Volume 11, Issue 3
Show Author Affiliations
Jung-Taek Oh, Texas A&M Univ. (United States)
Meng-Lin Li, Texas A&M Univ. (United States)
Hao F. Zhang, Texas A&M Univ. (United States)
Konstantin Maslov, Texas A&M Univ. (United States)
Lihong V. Wang, Texas A&M Univ. (United States)

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