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Journal of Biomedical Optics

Prediction of heat-induced collagen shrinkage by use of second harmonic generation microscopy
Author(s): Sung-Jan Lin; Wen Lo; Hsin-Yuan Tan; Jung-Yi Chan; Wei-Liang Chen; Shiou-Han Wang; Yen Sun; Wei-Chou Lin; Jau-Shiuh Chen; Chih-Jung Hsu; Jen-Wei Tiju; Hsin-Su Yu; Shiou-Hwa Jee; Chen-Yuan Dong
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Paper Abstract

Collagen shrinkage associated with denaturation from thermal treatment has a number of important clinical applications. However, individualized treatment is hindered by the lack of reliable noninvasive methods to monitor the process of collagen denaturation. We investigate the serial changes of collagen denaturation from thermal treatment of rat tail tendons at 58 °C by use of second harmonic generation (SHG) microscopy. We find that rat tail tendon shrinks progressively from 0 to 9 min of thermal treatment, and remains unchanged in length upon further thermal treatment. The SHG intensity also decreases from 0 to 9 min of thermal treatment and becomes barely detectable from further thermal treatment. Collagen shrinkage and the SHG intensity are well correlated in a linear model. In addition, SHG imaging reveals a tiger-tail-like pattern of collagen denaturation. The bands of denatured collagen progressively widen from increased thermal treatment and completely replace the adjacent bands of normal collagen after 9 min of thermal treatment. Our results show that collagen denaturation in rat tail tendon from thermal treatment is inhomogeneous, and that SHG intensity can be used to predict the degree of thermally induced collagen shrinkage. With additional development, this approach has the potential to be used in biomedical applications.

Paper Details

Date Published: 1 May 2006
PDF: 6 pages
J. Biomed. Opt. 11(3) 034020 doi: 10.1117/1.2209959
Published in: Journal of Biomedical Optics Volume 11, Issue 3
Show Author Affiliations
Sung-Jan Lin, National Taiwan Univ. Hospital (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Hsin-Yuan Tan, Chang Gung Memorial Hospital (Taiwan)
Jung-Yi Chan, Cathay General Hospital (Taiwan)
Wei-Liang Chen, National Taiwan Univ. (Taiwan)
Shiou-Han Wang, National Taiwan Univ. Hospital (Taiwan)
Yen Sun, National Taiwan Univ. (Taiwan)
Wei-Chou Lin, National Taiwan Univ. (Taiwan)
Jau-Shiuh Chen, National Taiwan Univ. Hospital (Taiwan)
Chih-Jung Hsu, National Taiwan Univ. Hospital (Taiwan)
Jen-Wei Tiju, National Taiwan Univ. (Taiwan)
Hsin-Su Yu, National Taiwan Univ. (Taiwan)
Shiou-Hwa Jee, National Taiwan Univ. Hospital (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

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