Share Email Print

Journal of Biomedical Optics

Analysis of long range correlations due to coherent light scattering from in-vitro cell arrays using angle-resolved low coherence interferometry
Author(s): John W. Pyhtila; Hongwei Ma; Andrew J. Simnick; Ashutosh Chilkoti; Adam Wax
Format Member Price Non-Member Price
PDF $20.00 $25.00

Paper Abstract

Angle-resolved low coherence interferometry (a/LCI) enables depth-resolved measurements of scattered light that can be used to recover subsurface structural information, such as the size of cell nuclei. Measurements of nuclear morphology, however, can be complicated by coherent scattering between adjacent cell nuclei. Previous studies have eliminated this component by applying a window filter to Fourier transformed angular data, based on the justification that the coherent scattering must necessarily occur over length scales greater than the cell size. To fully study this effect, results of experiments designed to test the validity of this approach are now presented. The a/LCI technique is used to examine light scattered by regular cell arrays, created using stamped adhesive micropatterned substrates. By varying the array spacing, it is demonstrated that cell-to-cell correlations have a predictable effect on light scattering distributions. These results are compared to image analysis of fluorescence micrographs of the cell array samples. The a/LCI results show that the impact of coherent scattering on nuclear morphology measurements can be eliminated through data filtering.

Paper Details

Date Published: 1 May 2006
PDF: 7 pages
J. Biomed. Opt. 11(3) 034022 doi: 10.1117/1.2209561
Published in: Journal of Biomedical Optics Volume 11, Issue 3
Show Author Affiliations
John W. Pyhtila, Duke Univ. (United States)
Hongwei Ma, Duke Univ. (United States)
Andrew J. Simnick, Duke Univ. (United States)
Ashutosh Chilkoti, Duke Univ. (United States)
Adam Wax, Duke Univ. (United States)

© SPIE. Terms of Use
Back to Top