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Journal of Biomedical Optics

Novel nontoxic mitochondrial probe for confocal fluorescence microscopy
Author(s): Silvia Versari; Anna Maria Villa; Alessandro Villa; Silvia M. Doglia; Giorgio A. Pagani; Silvia Bradamante
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Paper Abstract

We propose a 2,5-Bis[1-(4-N-methylpyridinium)ethen-2-yl)]-N-methylpyrrole ditriflate (PEPEP) as a novel nontoxic, nonpotentiometric mitochondrial probe for confocal fluorescence microscopy. PEPEP is a representative chromophore of a large family of heterocyclic fluorescent dyes that show fluorescence emission in aqueous media and great DNA affinity. We check its cytotoxicity and intracellular localization in mammalian and yeast cell cultures. We demonstrate that PEPEP is a very efficient dye for fluorescence confocal microscopy and a valuable alternative to the most frequently used mitochondrial stains.

Paper Details

Date Published: 1 May 2006
PDF: 4 pages
J. Biomed. Opt. 11(3) 034014 doi: 10.1117/1.2206173
Published in: Journal of Biomedical Optics Volume 11, Issue 3
Show Author Affiliations
Silvia Versari, ISTM Milano (Italy)
Anna Maria Villa, Univ. degli Studi Di Milano Bicocca (Italy)
Alessandro Villa, ISTM Milano (Italy)
Silvia M. Doglia, Univ. degli Studi di Milano Bicocca (Italy)
Giorgio A. Pagani, Univ. degli Studi di Milano-Bicocca (Italy)
Silvia Bradamante, ISTM Milano (Italy)

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