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Journal of Biomedical Optics

Investigation of living pancreas tumor cells by digital holographic microscopy
Author(s): Björn Kemper; Daniel D. Carl; Jürgen Schnekenburger; Ilona Bredebusch; Marcus Schäfer; Wolfram Domschke; Gert von Bally
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Paper Abstract

Digital holographic microscopy provides new facilities for contactless and marker-free quantitative phase contrast imaging. In this work, a digital holographic microscopy method for the integral refractive index determination of living single cells in cell culture medium is presented. Further, the obtained refractive index information is applied to full field thickness and shape determination of adherent pancreas tumor cells, as well as for analysis of drug-induced dynamic changes of a single cell's cytoskeleton. The results demonstrate that digital holographic microscopy is a quantitative phase contrast technique for living cells under conventional laboratory conditions.

Paper Details

Date Published: 1 May 2006
PDF: 8 pages
J. Biomed. Opt. 11(3) 034005 doi: 10.1117/1.2204609
Published in: Journal of Biomedical Optics Volume 11, Issue 3
Show Author Affiliations
Björn Kemper, Westfälische Wilhelms-Univ. Münster (Germany)
Daniel D. Carl, Univ. Münster (Germany)
Jürgen Schnekenburger, Univ. Münster (Germany)
Ilona Bredebusch, Univ. Münster (Germany)
Marcus Schäfer, Univ. Münster (Germany)
Wolfram Domschke, Univ. Münster (Germany)
Gert von Bally, Univ. Münster (Germany)


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