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Journal of Biomedical Optics

In-vivo imaging of tumor associated urokinase-type plasminogen activator activity
Author(s): Jong-Kai Hsiao; Benedict Law; Ralph Weissleder; Ching Hsuan Tung
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Paper Abstract

The ability to image tumor associated protease in vivo has biological and clinical implications. In the present study, we describe the development and validation of a urokinase-type plasminogen activator (uPA) sensitive fluorescence imaging probe. The activation of our probe is highly specific to uPA in both enzymatic and cellular-based assays. In two distinct in-vivo tumor models (human colon adenocarcinoma HT-29 and human fibrosarcoma HT-1080), the observed fluorescence changes correlate well with tumor associated uPA activity. The signal intensities of the tumors are about three-fold higher in animals with probe injections. Our results suggest a direct detection method for uPA activity in vivo and the approach can be used for monitoring tumor growth and development.

Paper Details

Date Published: 1 May 2006
PDF: 5 pages
J. Biomed. Opt. 11(3) 034013 doi: 10.1117/1.2204029
Published in: Journal of Biomedical Optics Volume 11, Issue 3
Show Author Affiliations
Jong-Kai Hsiao, National Taiwan Univ. Hospital (Taiwan)
Benedict Law, Massachusetts General Hospital (United States)
Ralph Weissleder, Massachusetts General Hospital (United States)
Ching Hsuan Tung, Massachusetts General Hospital (United States)

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