Share Email Print

Journal of Biomedical Optics

Noninvasive imaging of hemoglobin concentration and oxygenation in the rat brain using high-resolution photoacoustic tomography
Author(s): Xueding Wang; Xueyi Xie; Geng Ku; Lihong V. Wang; George Stoica
Format Member Price Non-Member Price
PDF $20.00 $25.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Simultaneous transcranial imaging of two functional parameters, the total concentration of hemoglobin and the hemoglobin oxygen saturation, in the rat brain in vivo is realized noninvasively using laser-based photoacoustic tomography (PAT). As in optical diffusion spectroscopy, PAT can assess the optical absorption of endogenous chromophores, e.g., oxygenated and deoxygenated hemoglobins, at multiple optical wavelengths. However, PAT can provide high spatial resolution because its resolution is diffraction-limited by photoacoustic signals rather than by optical diffusion. Laser pulses at two wavelengths are used sequentially to acquire photoacoustic images of the vasculature in the cerebral cortex of a rat brain through the intact skin and skull. The distributions of blood volume and blood oxygenation in the cerebral cortical venous vessels, altered by systemic physiological modulations including hyperoxia, normoxia, and hypoxia, are visualized successfully with satisfactory spatial resolution. This technique, with its prominent sensitivity to endogenous contrast, can potentially contribute to the understanding of the interrelationship between neural, hemodynamic, and metabolic activities in the brain.

Paper Details

Date Published: 1 March 2006
PDF: 9 pages
J. Biomed. Opt. 11(2) 024015 doi: 10.1117/1.2192804
Published in: Journal of Biomedical Optics Volume 11, Issue 2
Show Author Affiliations
Xueding Wang, Univ. of Michigan (United States)
Xueyi Xie, Texas A&M Univ. (United States)
Geng Ku, Texas A&M Univ. (United States)
Lihong V. Wang, Texas A&M Univ. (United States)
George Stoica, Texas A&M Univ. (United States)

© SPIE. Terms of Use
Back to Top