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Journal of Biomedical Optics

Simulation study of second-harmonic microscopic imaging signals through tissue-like turbid media
Author(s): Xiao-Yuan Deng; Xian-Ju Wang; Hanping Liu; Zhengfei Zhuang; Zhou-Yi Guo
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Paper Abstract

We establish, for the first time, a simulation model for dealing with the second-harmonic signals under a microscope through a tissue-like turbid medium, based on the Monte Carlo method. With this model, the angle-resolved distribution and the signal level η of second-harmonic light through a slab of the turbid medium are demonstrated and the effects of the thickness (d) of the turbid medium, the numerical aperture (NA) of the objective as well as the size (ρ) of the scatterers forming the turbid medium are explored. Simulation results reveal that the use of a small objective NA results in a narrow angle distribution but strong second-harmonic signals. A turbid medium consisting of large scattering particles has a strong influence on the angle distribution and the signal level η, which results in a low penetration limit for second-harmonic signals made up of ballistic photons. It is approximately 30 µm in our situation.

Paper Details

Date Published: 1 March 2006
PDF: 6 pages
J. Biomed. Opt. 11(2) 024013 doi: 10.1117/1.2191055
Published in: Journal of Biomedical Optics Volume 11, Issue 2
Show Author Affiliations
Xiao-Yuan Deng, South China Normal Univ. (China)
Xian-Ju Wang, South China Normal Univ. (China)
Hanping Liu, South China Normal Univ. (China)
Zhengfei Zhuang, South China Normal Univ. (China)
Zhou-Yi Guo, South China Normal Univ. (China)

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