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Journal of Biomedical Optics

Fluorescence microscopy imaging of electroperturbation in mammalian cells
Author(s): Yinghua Sun; P. Thomas Vernier; Matthew Behrend; Jingjing Wang; Mya Mya Thu; Martin A. Gundersen; Laura Marcu
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Paper Abstract

We report the design, integration, and validation of a fluorescence microscopy system for imaging of electroperturbation—the effects of nanosecond, megavolt-per-meter pulsed electric fields on biological cells and tissues. Such effects have potential applications in cancer therapy, gene regulation, and biophysical research by noninvasively disrupting intracellular compartments and inducing apoptosis in malignant cells. As the primary observing platform, an epifluorescence microscope integrating a nanosecond high-voltage pulser and a micrometer electrode chamber enable in situ imaging of the intracellular processes triggered by high electric fields. Using specific fluorescence molecular probes, the dynamic biological responses of Jurkat T lymphocytes to nanosecond electric pulses (nanoelectropulses) are studied with this system, including calcium bursts, the polarized translocation of phosphatidylserine (PS), and nuclear enlargement and chromatin/DNA structural changes.

Paper Details

Date Published: 1 March 2006
PDF: 10 pages
J. Biomed. Opt. 11(2) 024010 doi: 10.1117/1.2187970
Published in: Journal of Biomedical Optics Volume 11, Issue 2
Show Author Affiliations
Yinghua Sun, Univ. of Southern California (United States)
P. Thomas Vernier, Univ. of Southern California (United States)
Matthew Behrend, Univ. of Southern California (United States)
Jingjing Wang, Univ. of Southern California (United States)
Mya Mya Thu, Univ. of Southern California (United States)
Martin A. Gundersen, Univ. of Southern California (United States)
Laura Marcu, Univ. of California/Davis (United States)

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