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Journal of Biomedical Optics • Open Access

Detection of morphological markers of vulnerable atherosclerotic plaque using multimodal spectroscopy
Author(s): Obrad R. Scepanovic; Maryann Fitzmaurice; Joseph Gardecki; George O. Angheloiu; Samir Awasthi; Jason T. Motz; John R. Kramer; Ramachandra R. Dasari; Michael S. Feld

Paper Abstract

Vulnerable plaques, which are responsible for most acute ischemic events, are presently invisible to x-ray angiography. Their primary morphological features include a thin or ulcerated fibrous cap, a large necrotic core, superficial foam cells, and intraplaque hemorrhage. We present evidence that multimodal spectroscopy (MMS), a novel method that combines diffuse reflectance spectroscopy (DRS), intrinsic fluorescence spectroscopy (IFS), and Raman spectroscopy (RS), can detect these markers of plaque vulnerability. To test this concept, we perform an MMS feasibility study on 17 human carotid artery specimens. Following the acquisition of spectra, each specimen is histologically evaluated. Two parameters from DRS, hemoglobin concentration and a scattering parameter, are used to detect intraplaque hemorrhage and foam cells; an IFS parameter that relates to the amount of collagen in the topmost layers of the tissue is used to detect the presence of a thin fibrous cap; and an RS parameter related to the amount of cholesterol and necrotic material is used to detect necrotic core. Taken together, these spectral parameters can generally identify the vulnerable plaques. The results indicate that MMS provides depth-sensitive and complementary morphological information about plaque composition. A prospective in vivo study will be conducted to validate these findings.

Paper Details

Date Published: 1 March 2006
PDF: 9 pages
J. Biomed. Opt. 11(2) 021007 doi: 10.1117/1.2187943
Published in: Journal of Biomedical Optics Volume 11, Issue 2
Show Author Affiliations
Obrad R. Scepanovic, Massachusetts Institute of Technology (United States)
Maryann Fitzmaurice, Univ. Hospitals of Cleveland (United States)
Joseph Gardecki, Massachusetts Institute of Technology (United States)
George O. Angheloiu, Cleveland Clinic Foundation (United States)
Samir Awasthi, Massachusetts Institute of Technology (United States)
Jason T. Motz, Wellman Ctr. for Photomedicine (United States)
John R. Kramer, Massachusetts Institute of Technology (United States)
Ramachandra R. Dasari, Massachusetts Institute of Technology (United States)
Michael S. Feld, Massachusetts Institute of Technology (United States)

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