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Journal of Biomedical Optics

Collagen birefringence in skin repair in response to red polarized-laser therapy
Author(s): Daniela de Fátima Teixeira da Silva; Benedicto de Campos Vidal; Denise Maria Zezell; Telma M. T. Zorn; Silvia Cristina Núñez; Martha S. Ribeiro
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Paper Abstract

We use the optical path difference (OPD) technique to quantify the organization of collagen fibers during skin repair of full-thickness burns following low-intensity polarized laser therapy with two different polarization incidence vectors. Three burns are cryogenerated on the back of rats. Lesion L|| is irradiated using the electric field vector of the polarized laser radiation aligned in parallel with the rat's occipital-caudal direction. Lesion L is irradiated using the electric field vector of the polarized laser radiation aligned perpendicularly to the aforementioned orientation. Lesion C is untreated. A healthy area labeled H is also evaluated. The tissue samples are collected and processed for polarized light microscopy. The overall finding is that the OPD for collagen fibers depends on the electric field vector of the incident polarized laser radiation. No significant differences in OPDs are observed between L|| and H in the center, sides, and edges of the lesion. Lesions irradiated using the electric field vector of the polarized laser radiation aligned in parallel with the rat's occipital-caudal direction show higher birefringence, indicating that collagen bundles in these lesions are more organized.

Paper Details

Date Published: 1 March 2006
PDF: 6 pages
J. Biomed. Opt. 11(2) 024002 doi: 10.1117/1.2187418
Published in: Journal of Biomedical Optics Volume 11, Issue 2
Show Author Affiliations
Daniela de Fátima Teixeira da Silva, IPEN-CNEN/SP (Brazil)
Benedicto de Campos Vidal, Univ. Estadual de Campinas (Brazil)
Denise Maria Zezell, Instituto de Pesquisas Energéticas e Nucleares (Brazil)
Telma M. T. Zorn, Univ. de Sao Paulo (Brazil)
Silvia Cristina Núñez, IPEN-CNEN/SP (Brazil)
Martha S. Ribeiro, IPEN-CNEN/SP (Brazil)


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