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Journal of Biomedical Optics • Open Access

Automatic identification of biological microorganisms using three-dimensional complex morphology

Paper Abstract

We propose automated identification of microorganisms using three-dimensional (3-D) complex morphology. This 3-D complex morphology pattern includes the complex amplitude (magnitude and phase) of computationally reconstructed holographic images at arbitrary depths. Microscope-based single-exposure on-line (SEOL) digital holography records and reconstructs holographic images of the biological microorganisms. The 3-D automatic recognition is processed by segmentation, feature extraction by Gabor-based wavelets, automatic feature vector selection by graph matching, training rules, and a decision process. Graph matching combined with Gabor feature vectors measures the similarity of complex geometrical shapes between a reference microorganism and unknown biological samples. Automatic selection of the training data is proposed to achieve a fully automatic recognition system. Preliminary experimental results are presented for 3-D image recognition of Sphacelaria alga and Tribonema aequale alga.

Paper Details

Date Published: 1 March 2006
PDF: 8 pages
J. Biomed. Opt. 11(2) 024017 doi: 10.1117/1.2187017
Published in: Journal of Biomedical Optics Volume 11, Issue 2
Show Author Affiliations
Seokwon Yeom, Univ. of Connecticut (United States)
Bahram Javidi, Univ. of Connecticut (United States)

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