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Journal of Biomedical Optics

Real-time detection of caspase-2 activation in a single living HeLa cell during cisplatin-induced apoptosis
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Paper Abstract

Caspase-2 is important for the mitochondrial apoptotic pathway, however, the mechanism by which caspase-2 executes apoptosis remains obscure. We carry out the first measurements of the dynamics of caspase-2 activation in a single living cell by a FRET (fluorescence resonance energy transfer) probe. Two FRET probes are constructed that each encoded a CRS (caspase-2 or caspase-3 recognition site) fused with a cyan fluorescent protein (CFP) and a red fluorescent protein (DsRed) (CFP-CRS-DsRed). Using these probes, we found that during cisplatin-induced apoptosis, caspase-2 activation occurred more slowly than did activation of caspase-3; additionally, caspase-2 activation was initiated much earlier than that of caspase-3.

Paper Details

Date Published: 1 July 2006
PDF: 6 pages
J. Biomed. Opt. 11(2) 024011 doi: 10.1117/1.2187013
Published in: Journal of Biomedical Optics Volume 11, Issue 2
Show Author Affiliations
Juqiang Lin, Huazhong Univ. of Science and Technology (China)
Zhihong Zhang, Huazhong Univ. of Science and Technology (China)
Jie Yang, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Huazhong Univ. of Science and Technology (China)
Bifeng Liu, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)


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