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Journal of Biomedical Optics

Use of light absorbers to alter optical interrogation with epi-illumination and transillumination in three-dimensional cardiac models
Author(s): Venkat K. Ramshesh; Stephen B. Knisley
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Paper Abstract

Cardiac optical mapping currently provides 2-D maps of transmembrane voltage-sensitive fluorescence localized near the tissue surface. Methods for interrogation at different depths are required for studies of arrhythmias and the effects of defibrillation shocks in 3-D cardiac tissue. We model the effects of coloading with a dye that absorbs excitation or fluorescence light on the radius and depth of the interrogated region with specific illumination and collection techniques. Results indicate radii and depths of interrogation are larger for transillumination versus epi-illumination, an effect that is more pronounced for broad-field excitation versus laser scanner. Coloading with a fluorescence absorber lessens interrogated depth for epi-illumination and increases it for transillumination, which is confirmed with measurements using transillumination of heart tissue slices. Coloading with an absorber of excitation light consistently decreases the interrogated depths. Transillumination and coloading also decrease the intensities of collected fluorescence. Thus, localization can be modified with wavelength-specific absorbers at the expense of a reduction in fluorescence intensity.

Paper Details

Date Published: 1 March 2006
PDF: 12 pages
J. Biomed. Opt. 11(2) 024019 doi: 10.1117/1.2187012
Published in: Journal of Biomedical Optics Volume 11, Issue 2
Show Author Affiliations
Venkat K. Ramshesh, Univ. of North Carolina/Chapel Hill (United States)
Stephen B. Knisley, Univ. of North Carolina/Chapel Hill (United States)

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