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Journal of Biomedical Optics • Open Access

Effect of pulse duration on two-photon excited fluorescence and second harmonic generation in nonlinear optical microscopy
Author(s): Shuo Tang; Tatiana B. Krasieva; Zhongping Chen; Gabriel Tempea; Bruce Jason Tromberg

Paper Abstract

We have developed a multiphoton microscopy (MPM) system using a 12-fs Ti:sapphire laser with adjustable dispersion precompensation in order to examine the impact of pulse duration on nonlinear optical signals. The efficiencies of two-photon-excited fluorescence (TPEF) and second harmonic generation (SHG) were studied for various pulse durations, measured at the sample, ranging from ~400 fs to sub-20 fs. Both TPEF and SHG increased proportionally to the inverse of the pulse duration for the entire tested range. Because of improved signal-to-noise ratio, sub-20-fs pulses were used to enhance MPM imaging depth by approximately 160%, compared to 120-fs pulses, in human skin.

Paper Details

Date Published: 1 March 2006
PDF: 3 pages
J. Biomed. Opt. 11(2) 020501 doi: 10.1117/1.2177676
Published in: Journal of Biomedical Optics Volume 11, Issue 2
Show Author Affiliations
Shuo Tang, Univ. of California/Irvine (United States)
Tatiana B. Krasieva, Univ. of California/Irvine (United States)
Zhongping Chen, Univ. of California/Irvine (United States)
Gabriel Tempea, Femtolasers Produktions GmbH (Austria)
Bruce Jason Tromberg, Univ. of California/Irvine (United States)

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