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Journal of Biomedical Optics • Open Access

Two-photon excited autofluorescence imaging of human retinal pigment epithelial cells
Author(s): Meng Han; Almut Blindewald-Wittich; Frank G. Holz; Günter Giese; Markolf H. Niemz; Sarah Snyder; Hui Sun; Jiayi Yu; Michael Agopov; Olivier La Schiazza; Josef F. Bille

Paper Abstract

Degeneration of retinal pigment epithelial (RPE) cells severely impairs the visual function of retina photoreceptors. However, little is known about the events that trigger the death of RPE cells at the subcellular level. Two-photon excited autofluorescence (TPEF) imaging of RPE cells proves to be well suited to investigate both the morphological and the spectral characteristics of the human RPE cells. The dominant fluorophores of autofluorescence derive from lipofuscin (LF) granules that accumulate in the cytoplasm of the RPE cells with increasing age. Spectral TPEF imaging reveals the existence of abnormal LF granules with blue shifted autofluorescence in RPE cells of aging patients and brings new insights into the complicated composition of the LF granules. Based on a proposed two-photon laser scanning ophthalmoscope, TPEF imaging of the living retina may be valuable for diagnostic and pathological studies of age related eye diseases.

Paper Details

Date Published: 1 January 2006
PDF: 3 pages
J. Biomed. Opt. 11(1) 010501 doi: 10.1117/1.2171649
Published in: Journal of Biomedical Optics Volume 11, Issue 1
Show Author Affiliations
Meng Han, Ruprecht-Karls-Univ. Heidelberg (Germany)
Almut Blindewald-Wittich, Univ. Bonn (Germany)
Frank G. Holz, Univ. Bonn (Germany)
Günter Giese, Max-Planck-Institut für Medizinische Forschung (Germany)
Markolf H. Niemz, Univ. of Heidelberg (Germany)
Sarah Snyder, Ruprecht-Karls-Univ. Heidelberg (Germany)
Hui Sun, Ruprecht-Karls-Univ. Heidelberg (Germany)
Jiayi Yu, Ruprecht-Karls-Univ. Heidelberg (Germany)
Michael Agopov, Ruprecht-Karls-Univ. Heidelberg (Germany)
Olivier La Schiazza, Ruprecht-Karls-Univ. Heidelberg (Germany)
Josef F. Bille, Ruprecht-Karls-Univ. Heidelberg (Germany)

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