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Journal of Biomedical Optics

Calibration standards for multicenter clinical trials of fluorescence spectroscopy for in vivo diagnosis
Author(s): Nena Marín; Nicholas B. MacKinnon; Calum E. MacAulay; Sung K. Chang; Edward Neely Atkinson; Dennis D. Cox; Dan Serachitopol; Brian M. Pikkula; Michelle Follen; Rebecca R. Richards-Kortum
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Paper Abstract

In the context of clinical trials, calibration protocols for optical instruments that ensure measurement accuracy and the ability to carry out meaningful comparisons of data acquired from multiple instruments are required. A series of calibration standards and procedures are presented to assess technical feasibility of optical devices for cervical precancer detection. Measurements of positive and negative standards, and tissue are made with two generations of research grade spectrometers. Calibration accuracy, ability of standards to correct and account for changes in experimental conditions, and device components are analyzed. The relative frequency of measured calibration standards is investigated retrospectively using statistical analysis of trends in instrument performance. Fluorescence measurements of standards and tissue made with completely different spectrometers show good agreement in intensity and lineshape. Frequency of wavelength calibration standards is increased to every 2 h to compensate for thermal drifts in grating mount. Variations in illumination energy detected between standards and patient measurements require probe redesign to allow for simultaneous acquisition of illumination power with every patient measurement. The use of frequent and well-characterized standards enables meaningful comparison of data from multiple devices and unambiguous interpretation of experiments among the biomedical optics community.

Paper Details

Date Published: 1 January 2006
PDF: 14 pages
J. Biomed. Opt. 11(1) 014010 doi: 10.1117/1.2166389
Published in: Journal of Biomedical Optics Volume 11, Issue 1
Show Author Affiliations
Nena Marín, The Univ. of Texas at Austin (United States)
Nicholas B. MacKinnon, BC Cancer Agency (Canada)
Calum E. MacAulay, The BC Cancer Research Ctr. (Canada)
Sung K. Chang, Massachusetts General Hospital (United States)
Edward Neely Atkinson, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Dennis D. Cox, Rice Univ. (United States)
Dan Serachitopol, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Brian M. Pikkula, Rice Univ. (United States)
Michelle Follen, The Univ. of Texas M.D. Anderson Cancer Ctr. (United States)
Rebecca R. Richards-Kortum, Rice Univ. (United States)


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