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Journal of Biomedical Optics • Open Access

Evaluation of trapping efficiency of optical tweezers by dielectrophoresis
Author(s): Eirini I. Papagiakoumou; Dorel Pietreanu; Mersini I. Makropoulou-Loukogiannaki; Eugenia Kovacs; Alexander A. Serafetinides

Paper Abstract

A relatively new method for measuring optically induced forces on microparticles and cells, different from the conventional Brownian motion and viscous drag force calibration methods widely used, is introduced. It makes use of the phenomenon of dielectrophoresis for the calibration of optical tweezers through the dielectrophoretic force calculations. A pair of microelectrodes is fabricated by photolithography on a microscope slide and it is connected to a high-frequency generator. The calibration of the optical tweezers setup is performed by the manipulation of polystyrene beads and yeast cells. Calibration diagrams of the transverse forces versus power are deduced for different cell radii and numerical apertures of the objective lenses. The optical system and the related technique provide a fast and easy method for optical tweezers calibration.

Paper Details

Date Published: 1 January 2006
PDF: 8 pages
J. Biomed. Opt. 11(1) 014035 doi: 10.1117/1.2165176
Published in: Journal of Biomedical Optics Volume 11, Issue 1
Show Author Affiliations
Eirini I. Papagiakoumou, National Technical Univ. of Athens (Greece)
Dorel Pietreanu, Carol Davila Medical Univ. (Romania)
Mersini I. Makropoulou-Loukogiannaki, National Technical Univ. of Athens (Greece)
Eugenia Kovacs, Carol Davila Medical Univ. (Romania)
Alexander A. Serafetinides, National Technical Univ. of Athens (Greece)

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