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Journal of Biomedical Optics

Optical characterization of Pseudomonas fluorescens on meat surfaces using time-resolved fluorescence
Author(s): Alain Bouchard; Julie Frechette; Marcia L. Vernon; Jean-François Cormier; Rene M. Beaulieu; Réal Vallée; Akier Assanta Mafu
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Paper Abstract

A scanning optical system for the detection of bacteria on meat surfaces based on fluorescence lifetime and intensity measurements is described. The system detects autofluorescent light emitted by naturally occurring fluorophores in bacteria. The technique only requires minimal sample preparation and handling, thus the chemical properties of the specimen are preserved. This work presents the preliminary results obtained from a time-resolved fluorescence imaging system for the characterization of a nonpathogenic gram-negative bacteria, Pseudomonas fluorescens. Initial results indicate that the combination of fluorescence lifetime and intensity measurements provides a means for characterizing biological media and for detecting microorganisms on surfaces.

Paper Details

Date Published: 1 January 2006
PDF: 7 pages
J. Biomed. Opt. 11(1) 014011 doi: 10.1117/1.2162166
Published in: Journal of Biomedical Optics Volume 11, Issue 1
Show Author Affiliations
Alain Bouchard, Institut National d'Optique (Canada)
Julie Frechette, Institut National d'Optique (Canada)
Marcia L. Vernon, Institut National d'Optique (Canada)
Jean-François Cormier, Institut National d'Optique (Canada)
Rene M. Beaulieu, Institut National d'Optique (Canada)
Réal Vallée, Univ. Laval (Canada)
Akier Assanta Mafu, Food Research and Development Ctr. (Canada)

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