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Journal of Biomedical Optics

In vitro gene transfer to mammalian cells by the use of laser-induced stress waves: effects of stress wave parameters, ambient temperature, and cell type
Author(s): Mitsuhiro Terakawa; Shunichi Sato; Hiroshi Ashida; Kazuya Aizawa; Maki Uenoyama; Yoshinori Masaki; Minoru Obara
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Paper Abstract

Laser-mediated gene transfection has received much attention as a new method for targeted gene therapy because of the high spatial controllability of laser energy. We previously demonstrated both in vivo and in vitro that plasmid DNA can be transfected by applying nanosecond pulsed laser-induced stress waves (LISWs). In the present study, we investigated the dependence of transfection efficiency on the laser irradiation conditions and hence stress wave conditions in vitro. We measured characteristics of LISWs used for gene transfection. For NIH 3T3 cells, transfection efficiency was evaluated as functions of laser fluence and number of pulses. The effect of ambient temperature was also investigated, and it was found that change in ambient temperature in a specific range resulted in drastic change in transfection efficiency for NIH 3T3 cells. Gene transfection of different types of cell lines were also demonstrated, where cellular heating increased transfection efficiency for nonmalignant cells, while heating decreased transfection efficiency for malignant cells.

Paper Details

Date Published: 1 January 2006
PDF: 7 pages
J. Biomed. Opt. 11(1) 014026 doi: 10.1117/1.2160407
Published in: Journal of Biomedical Optics Volume 11, Issue 1
Show Author Affiliations
Mitsuhiro Terakawa, Keio Univ. (Japan)
Shunichi Sato, National Defense Medical College (Japan)
Hiroshi Ashida, National Defense Medical College (Japan)
Kazuya Aizawa
Maki Uenoyama, National Defense Medical College (Japan)
Yoshinori Masaki, National Defense Medical College (Japan)
Minoru Obara, Keio Univ. (Japan)

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