Share Email Print

Journal of Biomedical Optics • Open Access

Erythrocyte structure and dynamics quantified by Hilbert phase microscopy
Author(s): Gabriel Popescu; Takahiro Ikeda; Catherine Best; Kamran Badizadegan; Ramachandra R. Dasari; Michael S. Feld

Paper Abstract

We present a new quantitative method for investigating red blood cell morphology and dynamics. The instrument integrates quantitative phase microscopy with an inverted microscope, which makes it particularly suitable for the noninvasive assessment of live erythrocytes. In particular, we demonstrate the ability of this approach to quantify noninvasively cell volume and dynamic morphology. The subnanometer path-length sensitivity at the millisecond time scales is exemplified by measuring the hemoglobin flow out of the cell during hemolysis.

Paper Details

Date Published: 1 November 2005
PDF: 3 pages
J. Biomed. Opt. 10(6) 060503 doi: 10.1117/1.2149847
Published in: Journal of Biomedical Optics Volume 10, Issue 6
Show Author Affiliations
Gabriel Popescu, College of Optics and Photonics/Univ. of Central Florida (United States)
Takahiro Ikeda, Hamamatsu Photonics K.K. (Japan)
Catherine Best, Univ. of Illinois at Urbana-Champaign (United States)
Kamran Badizadegan, Harvard Medical School (United States)
Ramachandra R. Dasari, Massachusetts Institute of Technology (United States)
Michael S. Feld, Massachusetts Institute of Technology (United States)

© SPIE. Terms of Use
Back to Top