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Journal of Biomedical Optics • Open Access

Noninvasive measurement of cell volume changes by negative staining
Author(s): Miriam S. Droste; Stefan S. Biel; Lara Terstegen; Klaus-Peter Wittern; Horst Wenck; Roger Wepf

Paper Abstract

To maintain the intracellular concentration of ions and small molecules on osmotic challenges, nature has developed highly sophisticated transport systems for regulating water and ion content. An ideal measurement technique for volume changes of cells during osmotic challenges has to fulfil two requirements: it has to be osmotically inert, and it should allow online monitoring of cell volume changes. Here, a simple fluorescence microscopy-based approach is presented. Using fluorescein as a negative stain, it is possible to monitor cell volume changes without affecting the functionality of cell membranes and cell osmolarity. Measurement of Madine-Darby canine kidney (MDCK) cells after hypo- and hyperosmotic challenges reveals the main advantages of this approach: besides providing precise and reproducible quantitative data on reversible cell volume changes, the viability of the cells can be assessed directly by the appearance of stain in the cytoplasm. This becomes evident especially after hypo-osmotic challenge of glutaraldehyde-treated cells, which become leaky after fixation, followed by a massive volume change. This new approach represents a very sensitive measurement technique for cell volume changes resulting from water or ion flux, and thus seems to be an ideal tool for studying cell volume regulatory processes.

Paper Details

Date Published: 1 November 2005
PDF: 10 pages
J. Biomed. Opt. 10(6) 064017 doi: 10.1117/1.2138011
Published in: Journal of Biomedical Optics Volume 10, Issue 6
Show Author Affiliations
Miriam S. Droste, Beiersdorf AG (Germany)
Stefan S. Biel, Beiersdorf AG (Germany)
Lara Terstegen, Beiersdorf AG (Germany)
Klaus-Peter Wittern, Beiersdorf AG (Germany)
Horst Wenck, Beiersdorf AG (Germany)
Roger Wepf, Beiersdorf AG (Germany)

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