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Journal of Biomedical Optics

Planar fluorescence imaging using normalized data
Author(s): Vasilis Ntziachristos; Gordon M. Turner; Joshua Dunham; Stephen Windsor; Antoine Soubret; Jorge Ripoll; Helen A. Shih
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Paper Abstract

Fluorescence imaging of tissues has gained significant attention in recent years due to the emergence of appropriate reporter technologies that enable noninvasive sensing of molecular function in vivo. Two major approaches have been used so far for fluorescence molecular imaging, i.e., epi-illumination (reflectance) imaging and fluorescence molecular tomography. Transillumination is an alternative approach that has been employed for imaging tissues in the past and could be similarly beneficial for fluorescence molecular imaging. We investigate data normalization schemes in reflectance and transillumination mode and experimentally demonstrate that normalized transillumination offers significant advantages over planar reflectance imaging and over nonnormalized methods. Our observations, based on phantoms and on postmortem and in vivo mouse measurements display image quality improvement, superior depth sensitivity, and improved imaging accuracy over the nonnormalized methods examined. Normalized planar imaging retains implementation simplicity and could be used to improve on standard fluorescence reflectance imaging and as a simplified alternative to the more integrated and accurate tomographic methods.

Paper Details

Date Published: 1 November 2005
PDF: 8 pages
J. Biomed. Opt. 10(6) 064007 doi: 10.1117/1.2136148
Published in: Journal of Biomedical Optics Volume 10, Issue 6
Show Author Affiliations
Vasilis Ntziachristos, Massachusetts General Hospital (United States)
Gordon M. Turner, Massachusetts General Hospital (United States)
Joshua Dunham, Massachusetts General Hospital (United States)
Stephen Windsor, Massachusetts General Hospital (United States)
Antoine Soubret, Massachusetts General Hospital (United States)
Jorge Ripoll, Massachusetts General Hospital (United States)
Helen A. Shih, Massachusetts General Hospital (United States)

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