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Journal of Biomedical Optics • Open Access

Targeted DNA transfection into the mouse central nervous system using laser-induced stress waves
Author(s): Yasushi Sato; Kunio Takishima; Yasunari Kanda; Katsushige Mizuno; Yasuhiro Watanabe; Mitsuhiro Terakawa; Minoru Obara; Shogo Endo; Hidetoshi Ooigawa; Hiroshi Nawashiro; Shunichi Sato

Paper Abstract

We investigated the feasibility of gene transfer into the mouse central nervous system (CNS) by applying nanosecond pulsed laser-induced stress waves (LISWs). Intraventricular or hippocampal injection of a reporter gene [enhanced green fluorescent protein (EGFP)] followed by application of LISWs showed this method to be efficient in the CNS of newborn and adult mice. Cells expressing EGFP reside at least 3.5 mm from the surface of the tissue, while no apparent damage was detected. Additionally, expression of EGFP was limited to the area that was exposed to LISWs. Using this method, the formulation of plasmid DNA by cationic transfer reagent polyethylenimine proved to be effective for improving transfer efficiency into the CNS.

Paper Details

Date Published: 1 November 2005
PDF: 3 pages
J. Biomed. Opt. 10(6) 060501 doi: 10.1117/1.2128432
Published in: Journal of Biomedical Optics Volume 10, Issue 6
Show Author Affiliations
Yasushi Sato, National Defense Medical College (Japan)
Kunio Takishima, National Defense Medical College (Japan)
Yasunari Kanda, National Defense Medical College (Japan)
Katsushige Mizuno, National Defense Medical College (Japan)
Yasuhiro Watanabe, National Defense Medical College (Japan)
Mitsuhiro Terakawa, Keio Univ. (Japan)
Minoru Obara, Keio Univ. (Japan)
Shogo Endo, Okinawa Institute of Science and Technology (Japan)
Hidetoshi Ooigawa, National Defense Medical College (Japan)
Hiroshi Nawashiro, National Defense Medical College (Japan)
Shunichi Sato, National Defense Medical College (Japan)


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