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Journal of Biomedical Optics

Imaging of gene expression in living cells and tissues
Author(s): Robert H. Singer; David Lawrence; Ben Ovryn; John Condeelis
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Paper Abstract

It is possible to observe gene expression within single cells using a tetracycline inducible promoter for activation. Transcription can be observed by using a fluorescent fusion protein to bind nascent RNA. Ultimately, it is desirable to activate a reporter gene within a single cell with only photons. This is achieved by preparing a chemically altered transcription factor that is functionally unable to activate a reporter gene until it is exposed to photon excitation. We apply two-photon imaging to visualize tumor cells expressing a transgene and ultimately this approach will provide the means to activate a specific gene within a single cell within any tissue to ultimately observe its functional significance in situ.

Paper Details

Date Published: 1 September 2005
PDF: 9 pages
J. Biomed. Opt. 10(5) 051406 doi: 10.1117/1.2103032
Published in: Journal of Biomedical Optics Volume 10, Issue 5
Show Author Affiliations
Robert H. Singer, Albert Einstein College of Medicine (United States)
David Lawrence, Albert Einstein College of Medicine (United States)
Ben Ovryn, Albert Einstein College of Medicine (United States)
John Condeelis, Albert Einstein College of Medicine (United States)


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