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Journal of Biomedical Optics

Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low-coherence interferometry
Author(s): Adam Wax; John W. Pyhtila; Robert N. Graf; Ronald Nines; Charles W. Boone; Ramachandra R. Dasari; Michael S. Feld; Vernon E. Steele; Gary D. Stoner
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Paper Abstract

Angle-resolved low-coherence interferometry (a/LCI) is used to obtain quantitative, depth-resolved nuclear morphology measurements. We compare the average diameter and texture of cell nuclei in rat esophagus epithelial tissue to grading criteria established in a previous a/LCI study to prospectively grade neoplastic progression. We exploit the depth resolution of a/LCI to exclusively examine the basal layer of the epithelium, approximately 50 to 100 µm beneath the tissue surface, without the need for exogenous contrast agents, tissue sectioning, or fixation. The results of two studies are presented that compare the performance of two a/LCI modalities. Overall, the combined studies show 91% sensitivity and 97% specificity for detecting dysplasia, using histopathology as the standard. In addition, the studies enable the effects of dietary chemopreventive agents, difluoromethylornithine (DFMO) and curcumin, to be assessed by observing modulation in the incidence of neoplastic change. We demonstrate that a/LCI is highly effective for monitoring neoplastic change and can be applied to assessing the efficacy of chemopreventive agents in the rat esophagus.

Paper Details

Date Published: 1 September 2005
PDF: 10 pages
J. Biomed. Opt. 10(5) 051604 doi: 10.1117/1.2102767
Published in: Journal of Biomedical Optics Volume 10, Issue 5
Show Author Affiliations
Adam Wax, Duke Univ. (United States)
John W. Pyhtila, Duke Univ. (United States)
Robert N. Graf, Duke Univ. (United States)
Ronald Nines, The Ohio State Univ. (United States)
Charles W. Boone, National Cancer Institute (United States)
Ramachandra R. Dasari, Massachusetts Institute of Technology (United States)
Michael S. Feld, Massachusetts Institute of Technology (United States)
Vernon E. Steele, National Cancer Institute (United States)
Gary D. Stoner, The Ohio State Univ. (United States)

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