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Journal of Biomedical Optics

Noninvasive imaging of oral premalignancy and malignancy
Author(s): Petra B. B. Wilder-Smith; Tatiana B. Krasieva; Woong-Gyu Jung; Jun Zhang; Zhongping Chen; Kathryn Osann; Bruce Jason Tromberg
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Paper Abstract

Early detection of cancer and its precursors remains the best way to ensure patient survival and quality of life. Our specific aim is to test a multimodality approach to noninvasive diagnostics of oral premalignancy and malignancy. In the hamster cheek pouch model (120 hamsters), in vivo optical coherence tomography (OCT) and optical Doppler tomography (ODT) map epithelial, subepithelial, and vascular change throughout carcinogenesis. In vivo multiwavelength multiphoton (MPM) and second-harmonic generated (SHG) fluorescence techniques provided parallel data on surface and subsurface tissue structure, specifically collagen presence and structure, cellular presence, and vasculature. Images are diagnosed by two blinded, prestandardized investigators using a scale from 0 to 6 for all modalities. After sacrifice, histopathology is evaluated on a scale of 0 to 6. Imaging data are reproducibly obtained with good accuracy. Carcinogenesis-related structural and vascular changes are clearly visible to tissue depths of 2 mm. Sensitivity (OCT/ODT alone, 71 to 88%; OCT+MPM/SHG, 79 to 91%) and specificity (OCT alone, 62 to 83%; OCT+MPM/SHG, 67 to 90%) compare well with conventional techniques. Our conclusions are that OCT/ODT and MPM/SHG are promising noninvasive in vivo diagnostic modalities for oral dysplasia and malignancy.

Paper Details

Date Published: 1 September 2005
PDF: 8 pages
J. Biomed. Opt. 10(5) 051601 doi: 10.1117/1.2098930
Published in: Journal of Biomedical Optics Volume 10, Issue 5
Show Author Affiliations
Petra B. B. Wilder-Smith, Univ. of California/Irvine (United States)
Tatiana B. Krasieva, Univ. of California/Irvine (United States)
Woong-Gyu Jung, Univ. of California/Irvine (United States)
Jun Zhang, Univ. of California/Irvine (United States)
Zhongping Chen, Univ. of California/Irvine (United States)
Kathryn Osann, Univ. of California/Irvine (United States)
Bruce Jason Tromberg, Univ. of California/Irvine (United States)

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