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Journal of Biomedical Optics

Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements
Author(s): Michael Gösch; Hans Blom; Sylvain Anderegg; Kerstin Korn; Per Thyberg; Mona Wells; Theo Lasser; Rudolf Rigler; Anders Magnusson; Sverker Hard
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Paper Abstract

Dual-color cross-correlation spectroscopy allows the detection and quantification of labeled biomolecules at ultra-low concentrations, whereby the sensitivity of the assay correlates with the measurement time. We now describe a parallel multifocal dual-color spectroscopic configuration employing multiple avalanche photodiodes and hardware correlators. Cross-correlation curves are obtained from several dual-color excitation foci simultaneously. Multifocal dual-color excitation is achieved by splitting each of two laser beams (488 and 633 nm) into four sub-beams with the help of two 2×2 fan-out diffractive optical elements (DOEs), and subsequent superposition of the two sets of four foci. The fluorescence emission from double-labeled biomolecules is detected by two 2×2 fiber arrays.

Paper Details

Date Published: 1 September 2005
PDF: 7 pages
J. Biomed. Opt. 10(5) 054008 doi: 10.1117/1.2080707
Published in: Journal of Biomedical Optics Volume 10, Issue 5
Show Author Affiliations
Michael Gösch, École Polytechnique Fédérale de Lausanne (Switzerland)
Hans Blom, École Polytechnique Fédérale de Lausanne (Switzerland)
Sylvain Anderegg, École Polytechnique Fédérale de Lausanne (Switzerland)
Kerstin Korn, École Polytechnique Fédérale de Lausanne (Switzerland)
Per Thyberg, École Polytechnique Fédérale de Lausanne (Switzerland)
Mona Wells, École Polytechnique Fédérale de Lausanne (Switzerland)
Theo Lasser, École Polytechnique Fédérale de Lausanne (Switzerland)
Rudolf Rigler, École Polytechnique Fédérale de Lausanne (Switzerland)
Anders Magnusson
Sverker Hard


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