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Journal of Biomedical Optics

Optical sensor based on fluorescent quenching and pulsed blue LED excitation for long-term monitoring of dissolved oxygen in NASA space bioreactors
Author(s): Frank Gao; James Fay; Grace Mathew; Anthony S. Jeevarajan; Melody Anderson
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Paper Abstract

There is a need to monitor the concentration of dissolved oxygen (DO) present in the culture medium for NASA's space cell biology experiments, as well as in earth-based cell cultures. Continuous measurement of DO concentration in the cell culture medium in perfused bioreactors requires that the oxygen sensor provide adequate sensitivity and low toxicity to the cells, as well as maintain calibration over several weeks. Although there are a number of sensors for dissolved oxygen on the market and under development elsewhere, very few meet these stringent conditions. An in-house optical oxygen sensor (HOXY) based on dynamic fluorescent quenching of Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) chloride and a pulsed blue LED light source was developed in our laboratory to address these requirements. The sensing element consisted of the fluorescent dye embedded in a silicone matrix and coated onto a glass capillary. Photobleaching was minimized by a pulsed LED light source. The total noise in the sensor output is 2% and the sensor dynamic range is 0 to 200 mm Hg. The resolution of the sensor is 0.1 mm Hg at 50 mm Hg, and 0.25 mm Hg at 130 mm Hg, while the accuracy is 5%. The LED-based oxygen sensor exhibited stable performance and low drift, making it compatible for space-flight bioreactor systems.

Paper Details

Date Published: 1 September 2005
PDF: 6 pages
J. Biomed. Opt. 10(5) 054005 doi: 10.1117/1.2062427
Published in: Journal of Biomedical Optics Volume 10, Issue 5
Show Author Affiliations
Frank Gao, Wyle Labs. (United States)
James Fay, Texas Tech Univ. (United States)
Grace Mathew, Wyle Labs. (United States)
Anthony S. Jeevarajan, NASA Johnson Space Ctr. (United States)
Melody Anderson, NASA Johnson Space Ctr. (United States)

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