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Optical Engineering

Polychromatic image fusion algorithm and fusion metric for automatized microscopes
Author(s): Mario Alonso Bueno-Ibarra; Josué Álvarez-Borrego; Leonardo Acho; María Cristína Chávez-Sánchez
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Paper Abstract

We propose a new algorithm to determine the multifocus image fusion from several polychromatic images captured from the best focusing region where the best in focus image is included from a biological sample. This focusing region is built by including several images up and down starting from the Z position of the best image in focus. These captured RGB images are converted to YCbCr color space to have the color CbCr and intensity Y channels separated with the objective to preserve the color information of the best in focus image. Several approaches have been developed to fuse images, like those algorithms based on the wavelets transform, Laplacian, ratio, contrast or morphological pyramids selection, fusion by averaging, Bayesian methods, fuzzy sets, and artificial networks. However, this algorithm utilizes the Fourier approach by using the Y channel frequency content via analyzing the Fourier coefficients to retrieve the high frequencies to obtain the best possible characteristics of every captured image. After the completion of this process, we continue to construct the fused image with these coefficients and color information for the optimum in focus image in the YCbCr color space; as a result, we obtain a precise final RGB fused image.

Paper Details

Date Published: 1 September 2005
PDF: 7 pages
Opt. Eng. 44(9) 093201 doi: 10.1117/1.2048708
Published in: Optical Engineering Volume 44, Issue 9
Show Author Affiliations
Mario Alonso Bueno-Ibarra, Ctr. de Investigación Científica y de Educación Superior de Ensenada (United States)
Josué Álvarez-Borrego, Ctr. de Investigación Científica y de Educación Superior de Ensenada (Mexico)
Leonardo Acho, Ctr. de Investigación y Desarrollo de Tecnología Digital (United States)
María Cristína Chávez-Sánchez, Ctr. de Investigación en Alimentación y Desarrollo, A.C. (Mexico)

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