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Journal of Biomedical Optics • Open Access

Autofluorescence removal, multiplexing, and automated analysis methods for in-vivo fluorescence imaging
Author(s): James R. Mansfield; Kirk W. Gossage; Clifford C. Hoyt; Richard M. Levenson

Paper Abstract

The ability to image and quantitate fluorescently labeled markers in vivo has generally been limited by autofluorescence of the tissue. Skin, in particular, has a strong autofluorescence signal, particularly when excited in the blue or green wavelengths. Fluorescence labels with emission wavelengths in the near-infrared are more amenable to deep-tissue imaging, because both scattering and autofluorescence are reduced as wavelengths are increased, but even in these spectral regions, autofluorescence can still limit sensitivity. Multispectral imaging (MSI), however, can remove the signal degradation caused by autofluorescence while adding enhanced multiplexing capabilities. While the availability of spectral "libraries" makes multispectral analysis routine for well-characterized samples, new software tools have been developed that greatly simplify the application of MSI to novel specimens.

Paper Details

Date Published: 1 July 2005
PDF: 9 pages
J. Biomed. Opt. 10(4) 041207 doi: 10.1117/1.2032458
Published in: Journal of Biomedical Optics Volume 10, Issue 4
Show Author Affiliations
James R. Mansfield, CRI Inc. (United States)
Kirk W. Gossage, The Univ. of Arizona (United States)
Clifford C. Hoyt, Cambridge Research and Instrumentation, Inc. (United States)
Richard M. Levenson, CRI Inc. (United States)

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