Share Email Print

Journal of Biomedical Optics

Direct visualization of mRNA colocalization with mitochondria in living cells using molecular beacons
Author(s): Philip J. Santangelo; Nitin Nitin; Gang Bao
Format Member Price Non-Member Price
PDF $20.00 $25.00

Paper Abstract

The intracellular localization and specific organelle association of mRNA may reflect essential functions, stages, and stability of mRNA. We report the direct visualization of subcellular localization of K-ras and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNAs in live HDF cells using molecular beacons together with membrane-permeabilization and peptide-based delivery. Unexpectedly, we found that both K-ras and GAPDH mRNAs colocalize with mitochondria. Extensive control studies are performed, including the use of fluorescence in-situ hybridization (FISH), negative-control beacons, and the detection of colocalization of 28S ribosomal RNA with the rough endoplasmic reticulum (ER), suggesting that the mRNA localization and colocalization patterns observed in our study are true and specific. Our observation reveals intriguing subcellular associations of mRNA with organelles such as mitochondria, which may provide new insight into the transport, dynamics, and functions of mRNA and mRNA-protein interactions.

Paper Details

Date Published: 1 July 2005
PDF: 6 pages
J. Biomed. Opt. 10(4) 044025 doi: 10.1117/1.2011402
Published in: Journal of Biomedical Optics Volume 10, Issue 4
Show Author Affiliations
Philip J. Santangelo, Georgia Institute of Technology (United States)
Nitin Nitin, Georgia Institute of Technology (United States)
Gang Bao, Georgia Institute of Technology (United States)

© SPIE. Terms of Use
Back to Top