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Optical Engineering

Computer method for ELISA spot assay analysis
Author(s): Chih-Yang Lin; Yu-Tai Ching; Hsuen-Chin Chen; Betty A. Wu-Hsieh
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Paper Abstract

The ELISA (enzyme-linked immunosorbent assay) spot assay is a method widely used by immunologists to enumerate cytokine-producing cells within a specific cell population. The ELISA results are presented in an image containing numerous colored spots. We present a method to identify the spots in the image and report on important statistics regarding them. The proposed method employs color analysis in the CIE L*u*v* color space and matched filter technique. The system is trained to obtain a standard color for the spots and calculate the color differences between the spots and background in the L*u*v* space. Matched filters are then used to remove noise and enhance the spots in the color difference map. Intensity thresholding is applied to obtain a binary image in which the pixels in the spots have a grayscale of 1 while the grayscale of the other pixels is 0. A software system is implemented, based on this method, to help immunologists analyze the results obtained from the ELISA.

Paper Details

Date Published: 1 August 2005
PDF: 6 pages
Opt. Eng. 44(8) 083604 doi: 10.1117/1.2009767
Published in: Optical Engineering Volume 44, Issue 8
Show Author Affiliations
Chih-Yang Lin, National Chiao Tung Univ. (Taiwan)
Yu-Tai Ching, National Chiao Tung Univ. (Taiwan)
Hsuen-Chin Chen, National Taiwan Univ. (Taiwan)
Betty A. Wu-Hsieh, National Taiwan Univ. (Taiwan)

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