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Journal of Biomedical Optics

Investigation of red blood cell antigens with highly fluorescent and stable semiconductor quantum dots
Author(s): Patricia M. A. Farias; Beate Saegesser Santos; Frederico Duarte Menezes; Ricardo C. Ferreira; Maria de Lourdes Barjas-Castro; Vagner Castro; Paulo Roberto Moura Lima; Adriana Fontes; Carlos Lenz Cesar
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Paper Abstract

We report a new methodology for red blood cell antigen expression determination by a simple labeling procedure employing luminescent semiconductor quantum dots. Highly luminescent and stable core shell cadmium sulfide/cadmium hydroxide colloidal particles are obtained, with a predominant size of 9 nm. The core-shell quantum dots are functionalized with glutaraldehyde and conjugated to a monoclonal anti-A antibody to target antigen-A in red blood cell membranes. Erythrocyte samples of blood groups A+, A2+, and O+ are used for this purpose. Confocal microscopy images show that after 30 min of conjugation time, type A+ and A2+ erythrocytes present bright emission, whereas the O+ group cells show no emission. Fluorescence intensity maps show different antigen expressions for the distinct erythrocyte types. The results obtained strongly suggest that this simple labeling procedure may be employed as an efficient tool to investigate quantitatively the distribution and expression of antigens in red blood cell membranes.

Paper Details

Date Published: 1 July 2005
PDF: 4 pages
J. Biomed. Opt. 10(4) 044023 doi: 10.1117/1.1993257
Published in: Journal of Biomedical Optics Volume 10, Issue 4
Show Author Affiliations
Patricia M. A. Farias, Univ. Federal de Pernambuco (Brazil)
Beate Saegesser Santos, Univ. Federal de Pernambuco (Brazil)
Frederico Duarte Menezes, Univ. Federal de Pernambuco (Brazil)
Ricardo C. Ferreira, Univ. Federal de Pernambuco (Brazil)
Maria de Lourdes Barjas-Castro, Univ. Estadual de Campinas (Brazil)
Vagner Castro, Univ. Estadual de Campinas (Brazil)
Paulo Roberto Moura Lima, Univ. Estadual de Campinas (Brazil)
Adriana Fontes, Univ. Estadual de Campinas (Brazil)
Carlos Lenz Cesar, Univ. Estadual de Campinas (Brazil)

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